The Dutch rider had stamped her authority on the race during Saturday's Stage 9, winning on Monte Zoncolan to open up a big gap in the General Classification.
And the 35-year-old made sure there wasn't to be an upset by dominating the final stage to seal her title in style.
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The highlight of Van Vleuten's race was her ascent of Zoncolan, which was making only its second appearance in the Giro Rosa and the first using the tougher climb from Ovaro.
Speaking after the stage, Van Vleuten said:
Today was epic on the Zoncolan, but I loved it. I was looking forward to this day and of course defending the GC and the Maglia Rosa was my primary objective, but I also wanted to win this stage really, really badly. It’s awesome that the Queen Stage was organised on such a famous climb like Monte Zoncolan, it’s really good for women’s cycling and makes the day unforgettable. It is such a hard climb, so steep that it was difficult to put pressure on the front wheel. I didn’t recon the climb beforehand, but I knew it was steep and I actually had something left to attack Ashleigh Moolman-Pasio because I really wanted to win and the last few hundred metres there were so many people clapping and cheering, it was so special, and I had a little bit of time to enjoy the moment.
Ashleigh Moolman and fellow Mitchelton-Scott rider Amanda Spratt completed the Giro Rosa podium.
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