The Australian put in the standout performance of the penultimate session of the TISSOT UCI Track World Cup, as the Great Britain women's team pursuit squad also impressed with a comfortable ride to make the gold medal race, this afternoon.
There were signs of promise for the home fans in the kilo too, as 100% ME's Callum Skinner qualified second fastest.
But it was Glaetzer who topped the charts with the only time under one minute - clocking 59.970s, thought to be the fastest ever time at sea level - as Skinner recorded an effort of 1:00.868.
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Ayrton de Pauw, Sebastien Vigier and Jonathan Wale will also ride this afternoon's final, as will Eric Engler, David Sojka and Theo Bos.
Earlier in the session Great Britain's women showed that anything the men can do, they can do better as their quartet breezed through the team pursuit first round with a win over Belgium.
Emily Kay and Manon Lloyd came in to replace Katie Archibald and Elinor Barker, and they combined with Neah Evans and Emily Nelson to set a time of 4:23.780 - almost two seconds faster than their opponents in the final, Italy.
The bronze medal final will see Japan take on Germany after they set the fastest times of the remaining six teams.
But Team Breeze's Rebecca Raybould was left disappointed after the young squad failed to improve on their promising time in qualifying.
"We were a bit disappointed to not get into the bronze medal final - we were hoping to do a better ride than yesterday, but at the end of the day we're still here for a learning experience and it's still been really good," said the 19-year-old.
"We did a much faster time than we were expecting in qualifying. We were really pleased with that and when all the teams around us did very similar times we were hopeful that a going a bit faster would push us up - obviously on Sunday that wasn't meant to be."
The usual suspects eased through to the second round of the men's keirin as Britain's Joe Truman, world champion Azizul Awang, former rainbow jersey wearer Joachim Eilers and sprint semi-finalist Matthias Buchli all progressed.
Joining them will be Andrii Vynokurov, Leandro Bottasso, Patryk Rajkowski and Glaetzer, while Juan Peralta, Quentin Lafargue, Yudai Nitta and Jordan Castle were all forced into the repechages.
The final event of the morning saw the women's sprint semi-final lineups confirmed for this afternoon, with Kristina Vogel going in search of a golden hat-trick.
The German world champion already has two golds in her kit bag this weekend, and is looking to make it a treble for the second weekend in a row.
She defeated Olena Starikova in straight rides and will go on to face Shanne Braspennincx in the semi-finals after the Dutchwoman beat China's Guo Yufang.
And the second semi-final will see fellow Dutch rider Laurine van Riessen go up against Russia's Anastasiia Vionova, after they came past Simona Krupeckaite and Madelyn Godby, respectively.
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