The 22-year-old had been hoping to win his maiden caps at full international level.
Maddison is the second midfielder to withdraw from the squad for the trips to Czech Republic and Bulgaria and once again, there will be no replacement called up.
World Cup
Walker and Maddison out of England opener against Iran, confirms Southgate
20/11/2022 AT 15:56
England can seal their place at Euro 2020 on Friday with victory over the Czechs in Prague.
Gareth Southgate has looked to shuffle his pack ahead of this round of qualifying, with several familiar names such as Dele Alli, Eric Dier and Kyle Walker left out.
World Cup
'Precautionary, he's okay' - Rodgers allays Maddison injury fears
12/11/2022 AT 16:11
World Cup
Bullseye! An England World Cup squad everyone* agrees on – The Warm-Up
11/11/2022 AT 08:22