The Renault e.dams driver charged from 14th to fifth in the first half of the Tempelhof double-header.
Post-race checks then revealed that all four tyres on both of his cars were below the minimum pressure stipulated by tyre supplier Michelin.
Minimum tyre pressure was set at 1.60 bar - the tyres on Buemi's first car came in at 1.46, 1.57, 1.54 and 1.52 bar respectively, while on the second car the pressures were 1.54, 1.54, 1.59 and 1.53 bar.
This breached Article 25.11 of Formula E's sporting regulations and Buemi has been disqualified as a result.
That cuts his championship lead from 32 points to 22, after nearest rival Lucas di Grassi qualified on pole position and finished second.
It also promotes Nico Prost to fifth in the race results and elevates Daniel Abt, Sam Bird, Jean-Eric Vergne, Maro Engel and (into the points) Oliver Turvey.
Renault e.dams maintains a healthy lead in the teams' championship, although its advantage over Abt Audi Sport drops from 66 points to 54.