Ukrainian Dayana Yastremska's second appeal for her provisional doping suspension to be lifted has been denied by the chair of the independent tribunal convened to hear her case, the International Tennis Federation said in a statement.
Yastremska, 20, was suspended in January after the World Anti-Doping Agency (WADA) found a banned substance in an out-of-competition urine sample she submitted.
The ITF had denied Yastremska's earlier application to have the suspension lifted, prompting the world number 31 to approach the Court of Arbitration for Sport which dismissed her appeal in February.
ATP Tokyo
Highlights: Top-seed Ruud crashes out in first round to Munar in Tokyo
That decision left Yastremska ineligible to play in the Australian Open, the year's first Grand Slam, after the player had travelled to the country in the hope that her suspension would be lifted.
Yastremska had provided her urine sample in November and a WADA laboratory in Montreal found the presence of mesterolone metabolite, an anabolic agent on its Prohibited List.
She has denied using performance-enhancing drugs and said the positive test was the result of a "contamination event".
'Crazy' - Alcaraz gives reaction to topping world rankings with hero Nadal second
ATP Tokyo
Highlights: Coric knocks out Kokkinakis to reach Japan Open second round