The Czech had been due to play in the first round at Roland Garros on Monday morning against Romanian player Sorana Cirstea.
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However, she announced on Twitter that she was unable to take part due to injury, saying:
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"I'm so disappointed to have to announce my withdrawal from Roland Garros. I've had pain in my left forearm for a few weeks and last night an MRI confirmed a grade two tear which unfortunately could get a lot worse if I play today.

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"Two years ago, I made my comeback at Roland Garros, so I'm truly sad not to be able to play here this year. It is a really tough decision to make, but I wish the tournament all the best for a successful event and can't wait to be back in 2020."
Kvitova has been replaced in the draw by lucky loser Kaja Juvan of Slovenia.
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