Rooney, who served a one-match suspension by missing D.C. United's game against Philadelphia Union, will now miss Saturday's match against Montreal Impact.
Rooney's red card against the Red Bulls came after VAR spotted the infraction, which the referee had not seen in a crowded penalty area at a corner kick.
League Cup
Rooney: Sacking Mourinho before League Cup final 'crazy'
20/04/2021 AT 07:45
In his second season with D.C. United, Rooney will leave at the end of this campaign to join English Championship team Derby County as a player and assistant coach in January.
D.C. United have lost their past three matches and are fifth in the 12-team Eastern Conference with 39 points from 29 matches.
Premier League
‘What a player!’ - Saka is Arsenal's best player, says Wilshere
22/01/2021 AT 21:47
Premier League
Pogba: Cavani gives United quality not offered by Martial or Rashford
22/01/2021 AT 09:39